You can try: http://toolkit.tuebingen.mpg.de/hhpred
this gives you a nice domain border prediction based on analog. structures. Best wishes Kornelius On Tue, 20 May 2008 19:34:39 +0200 Jayashankar <[EMAIL PROTECTED]> wrote: > Dear friends and scientists, > > (A pre-Structural biological question.) > > I Have a multidomain protein , I know the domain boundaries, > But am still not that rational to what residues a construct should start or > end? > > But I have learned from people that changing one residue changes the fate of > the construct. > > > I will be greatful for the insights from the scientific community. > I dont want to do any Directed evolution approach. > > -- > S.Jayashankar > Research Student > Institute for Biophysical Chemistry > Hannover Medical School > Germany ---------------------------------------------- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [EMAIL PROTECTED] Tel -49 7071 601 323 Fax -49 7071 601 349
