Perhaps it should also be noted here that the long crystal axis should be
oriented close to parallel to phi, in order to minimize overlaps. I have
heard anecdotal evidence (anyone else?) that this long axis, in plate
crystals, is usually normal to the surface of the plates.
Perpendicularly-bent/folded loops, in combination with goniometer
adjustments, can used to acheive this. The plate is then shot through the
edge from all directions. Do others agree with this? It makes sense to me
theoretically and in my own experience, anyway.
Jacob
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [EMAIL PROTECTED]
*******************************************
----- Original Message -----
From: <[EMAIL PROTECTED]>
To: <CCP4BB@JISCMAIL.AC.UK>
Sent: Thursday, May 22, 2008 9:47 AM
Subject: [ccp4bb] Mosflm : data process of crystal with huge unit cell
Dear All
I am processing data from a crystal for a large macromolecular complex
with mosflm. Cell dimensions are around 120 x 150 x 650, with a p222
spacegroup. To avoid overlaps, we have collected data with a oscillation
of 0.1 degrees.
When I try to process the data with mosflm, mosaicity decreases along the
processing to a very low values (0.05 to 0.1 depending on the images). The
result is that I miss a lot of spots from the images.
I would appreciate any help regarding :
1.- What's is the best strategy to process such a dataset ?
2.- Which are the critical parameters in mosflm to avoid these problems,
and how to modify them ?
3.- and finally, can I use this data or your advice is to try to get
crystals in a different spacegroup ?
Many thanks
Best regards
Francisco
-----------------------------------------
Francisco J. Enguita, Ph.D.
Macromolecular Crystallography Laboratory
ITQB
EAN, Av. da República
2781-901 Oeiras
Portugal
Phone : +351-21-4469663
Fax : +351-21-4433644
E-mail : [EMAIL PROTECTED]
-----------------------------------------
