according to literature,see below and references http://www.px.nsls.bnl.gov/courses/papers/ZD_EG_papers.html, it is not clear that liq. propane plunged item would cool faster. (whilst i havent tested this)...
Would anyone have actual experimental data with protein crystals on the hyperquenching suggested by Warkentin, V. Berejnov, and R. E. Thorne, J. Appl. Cryst. (2006) 39, 805-811. (no diffraction data in the paper). in particular with small samples. thanks, Tommi Quoting Petr Leiman <[EMAIL PROTECTED]>: > >> yes you are right, but I assumed if people see a cloud of condensed > >> fog over their LN2 bath they should remove that by > >> a) filling up the bowl completely e.g. some LN2 drips out of the bowl > >> b) blow the fog away before you dip > > I think the original poster meant the relatively low heat conduction of > liquid N2, which causes boiling around the crystal immediately after > plunging. > > The best way to freeze things is to put a small container of liquid > ethane > or propane into a liquid N2 bowl, and plunge into the ethane/propane > (this > methods was suggested earlier). > > Petr > > -- Tommi Kajander, Ph.D. Macromolecular X-ray Crystallography Research Program in Structural Biology and Biophysics Institute of Biotechnology P.O. Box 65 (Street address: Viikinkaari 1, 4th floor) University of Helsinki FIN-00014 Helsinki, Finland Tel. +358-9-191 58903 Fax +358-9-191 59940
