Hi Ji,
Our lab has had good luck using sodium malonate to cryoprotect salt-
grown crystals. See:
Acta Cryst. (2003). D59, 2356-2358
Malonate: a versatile cryoprotectant and stabilizing solution for salt-
grown macromolecular crystals
T. Holyoak, T. D. Fenn, M. A. Wilson, A. G. Moulin, D. Ringe and G. A.
Petsko
Best,
Wally
On Jun 24, 2008, at 2:14 AM, Ji lee wrote:
Dear,
I have a crystal diffracted anisotrophically. I tested with a few
different cryo conditions like oil, glycerol in different
concentration to get a better data but these conditions didn't help
any.
Using capillary method improved the diffraction (isotrophic) but the
crystal couldn't survive during the data collection.
Is there any methods or cryo conditions I can use to improve my
diffraction data?
This crystal grew in 2.5M Ammonium Sulfate.
Thank you so much.
Ji
Walter R.P. Novak, Ph.D.
Postdoctoral Fellow
Rosenstiel Basic Medical Research Center
Brandeis University
415 South St. MS 029
Waltham, MA 02454-9110
Phone: (781) 736-4944
Fax: (781) 736-2405
