Hi,
I have a very good native data set. However, the selenomethionyl
crystal has a different space group and always suffers from radiation
damage. A three-wavelength data set was still collected, and after
phasing in SHARP, some features can be seen but the map is not good
enough for tracing. Then I did some heavy atom soaking with the
native crystals and collected data at home, however, the crystal was
again converted into the same space group as the selenomethionyl
crystal. At lease 4 Hg sites can be found both by isomorphous
difference Fourier calculated using the partial Se-MAD phase and by
isomorphous difference Patterson, suggesting they should be real. Now
with these information, what's the best way to do the phasing in
SHARP, or in some other programs?
I hope I have made myself clear; and I would like to supply more
details. Any suggestion will be highly appreciated.
Best regards,
Junyu
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Junyu Xiao
Department of Biological Chemistry,
University of Michigan
Lab address:
3163 Life Sciences Institute
University of Michigan
210 Washtenaw Avenue
Ann Arbor, MI, 48109-2216
Phone: 734-615-2078
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