It should be pretty straightforward to figure that out by freezing and shooting loops with mother liquor only. You can easily fine-tune by including any additional buffer components you may have. Bert van den Berg University of Massachusetts Medical School Program in Molecular Medicine Biotech II, 373 Plantation Street, Suite 115 Worcester MA 01605 Phone: 508 856 1201 (office); 508 856 1211 (lab) e-mail: [EMAIL PROTECTED] http://www.umassmed.edu/pmm/faculty/vandenberg.cfm
________________________________ From: CCP4 bulletin board on behalf of Tjaard Pijning Sent: Tue 7/22/2008 8:41 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Cryo with succinate I have crystals grown from 1 M sodium succinate pH 7 and have been searching for suitable cryo conditions. Is succinate in itself already a cryoprotectant (maybe at somewhat higher concentration) or should I add e.g. glycerol ? Any experience or ideas are welcome. Thanks in advance, <https://mail.umassmed.edu/Bert.Vandenberg/Drafts/RE:%20%5Bccp4bb%5D%20Cryo%20with%20succinate-2.EML/1_multipart/logo.jpg> Ing. Tjaard Pijning Protein Crystallography Group University of Groningen Nijenborgh 4 9747 AG Groningen The Netherlands Tel. (31)(0)50 363 4385
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