Re: [ccp4bb] Cryo with succinate

[Alastair McEwen]

I have recently been working with crystals grown 
from 1M sodium succinate at pH 7.5 and I have 
found that 20% ethylene glycol worked well as a 
cryoprotectant. At concentrations below this we 
had ice formation. We did not do an exhaustive 
search for a cryo but found that we had very poor 
diffraction with glycerol, parrafin oil and various Fomblin oils.

Hope this helps,
Alastair


Le 14:41 22/07/2008,Tjaard Pijning écrit:
I have crystals grown from 1 M sodium succinate 
pH 7 and have been searching for suitable cryo conditions.
Is succinate in itself already a cryoprotectant 
(maybe at somewhat higher concentration) or should I
add e.g. glycerol ? Any experience or ideas are welcome.

Thanks in advance,


[]
   Ing. Tjaard Pijning

Protein Crystallography Group
University of Groningen
Nijenborgh 4
9747 AG Groningen
The Netherlands
Tel. (31)(0)50 363 4385




Dr. Alastair McEwen
Département de Biologie et Génomique Structurales
IGBMC, 1 rue Laurent Fries, BP10142
67404 ILLKIRCH, France
Tel:  +33 (0)3 88 65 57 73
Fax: +33 (0)3 88 65 32 76
email: [EMAIL PROTECTED] <<attachment: 121fc34.jpg>>
Dr. Alastair McEwen
Département de Biologie et Génomique Structurales
IGBMC, 1 rue Laurent Fries, BP10142
67404 ILLKIRCH, France
Tel:  +33 (0)3 88 65 57 73
Fax: +33 (0)3 88 65 32 76
email: [EMAIL PROTECTED]