Mark, There are two very easy alternatives. First is the Bradford Coomassie Dye binding assay. You can buy a kit from Biorad at the following link: http://www.bio-rad.com/B2B/BioRad/product/br_category.jsp?BV_SessionID=@@@@0349900713.1219329576@@@@&BV_EngineID=ccchadeemgdfflfcfngcfkmdhkkdfll.0&categoryPath=%2fCatalogs%2fLife+Science+Research%2fSample+Quantitation%2fProtein+Assay+Kits+and+Cuvettes%2fBio-Rad+Protein+Assay&divName=Corporate&loggedIn=false&lang=English&country=HQ&catLevel=5&catOID=-12777&isPA=false&serviceLevel=Lit+Request&searchStr=coomassie&cateName=Ordering+Information
Second is the BCA assay which can be purchased from Pierce (now bought out by Thermo/Fisher): http://www.piercenet.com/Products/Browse.cfm?fldID=02020101 Cheers, Jim On Thu, Aug 21, 2008 at 6:54 AM, Mark Hilge <[EMAIL PROTECTED]> wrote: > Dear all, > > I would be glad to hear what (simple) method I should use to determine > protein concentrations as accurately as possible. Presently, I'm measuring > absorption at 280nm with a nanodrop device. I either have 0 or 1 tryptophan > and no activity test. > > Many thanks in advance! > > Best regards, > > Mark > > Mark Hilge > Protein Biophysics > NCMLS 274 > 3rd floor M850.03.035 > Geert Grooteplein 28 > 6525 GA Nijmegen > The Netherlands > > http://www.mark-hilge.com > > Phone: 0031 24 36 10 525 > > > > Het UMC St Radboud staat geregistreerd bij de Kamer van Koophandel in het > handelsregister onder nummer 41055629. > The Radboud University Nijmegen Medical Centre is listed in the Commercial > Register of the Chamber of Commerce under file number 41055629. > -- Jim Fairman Graduate Research Assistant Department of Biochemistry, Cellular, and Molecular Biology (BCMB) University of Tennessee -- Knoxville 216-368-3337 [EMAIL PROTECTED] [EMAIL PROTECTED]
