Dear All Sorry for non-crystallography query. I am working on DNA binding protein, while mixing DNA with protein for preparing Protein-DNA complex for crystallization, protein is precipitating. pI of the protein is 9.3 and in 15 mM HEPES 7.0, 150mM NaCl and 5% glycerol. Concentration of the protein used for mixing with DNA is 8 mg/mL. DNA to protein molar ratio is 1.2. Please advise me how to prevent precipitation. Is changing of buffer pH and adding divalent cation like MgCl2 can help in preventing precipitation? Thanking You Rajakumara
E. Rajakumara Postdoctoral Fellow Strcutural Biology Program Memorial Sloan-Kettering Cancer Center New York-10021 NY 001 212 639 7986 (Lab) 001 917 674 6266 (Mobile) Get your preferred Email name! Now you can @ymail.com and @rocketmail.com. http://mail.promotions.yahoo.com/newdomains/aa/