[ccp4bb] Quikchange cloning: Insert length

[Raji Edayathumangalam]

Hi Folks,

Sorry for the non-xtallo posting.
I am curious to hear what is the longest insert anyone has cloned  
using a modification of the Quikchange cloning strategy. Basically,  
ligation-independent cloning by strapping on homologous regions of  
the vector onto the primers which also generate the initial PCR  
product. I plan to proceed with my insert which is ~ 2kb and am  
curious to get some feedback if you have successfully cloned inserts  
> 1.5kb using the above strategy.

Many thanks.
Raji