Thank you Patrick for the reply, as well to another person who has replied directly to me.
Please provide with examples if you know of any (say a reference or a PDB id ), as it would allow for comparison between published results and my own crystallization system. To answer your points: The crystallization conditions in the case at hand are as follow : the precipitant is PEG, the amount of salt is relatively low (0.1 M buffer + some NaCl etc.), and the pH is 8.0. The SEC experimental conditions are not too far away from the crystallization conditions except, of course, for the presence of PEG. Thierry From: Patrick Loll [mailto:[EMAIL PROTECTED] Sent: Monday, December 01, 2008 05:59 PM To: Fischmann, Thierry Cc: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] O/T: can a protein which dimerizes in solution crystallize as a monomer? depends on the crystallization conditions (and the SEC conditions). E.g., if the crystals grow in 4 M salt at pH 3, maybe that disrupts a complex that forms under more physiological conditions On 1 Dec 2008, at 5:47 PM, Fischmann, Thierry wrote: Dear fellow crystallographers, This is a question which is not CCP4-related. Is anybody aware of a protein which is known to be a dimer in solution (say by SEC), and yet crystallizes as a monomer? Wouldn't the high concentration in the crystallization drop further favor dimerization? In other words, if a protein crystallizes as a monomer, can I conclude that it does not form biologically relevant dimers in solution? Thank you in advance for your replies. Thierry ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. ------------------------------------------------------------------------ --------------- Patrick J. Loll, Ph. D. Professor of Biochemistry & Molecular Biology Director, Biochemistry Graduate Program Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102-1192 USA (215) 762-7706 [EMAIL PROTECTED] ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete.
