the tandem KH domain of FMRP crystallized as a very convincing dimer
(valverde et al 2007), but is a monomer in solution, although it is
not the whole protein but just two domains of it.. anyway, i would
think these ar much more common than the other way around.
Tommi
Quoting "Poul Nissen" <[EMAIL PROTECTED]>:
I would say that all crystals represent hyper-oligomeric structures,
but never mind, I know what you mean ;-)
the E. coli EF-Tu:EF-Ts complex is a good example - the structure
clearly indicates an (EF-Tu:EF-Ts)2 dimer, and the T. thermophilus
EF-Tu:EF-Ts is even a disulphide-linked dimer.
However, all solution studies indicate that the E.coli EF-Tu:EF-Ts
complex is in fact a monomeric complex.
Poul
On 11/12/2008, at 17.09, Santarsiero, Bernard D. wrote:
In parallel with the discussion around this off-CCP4-topic, are they any
good examples of the opposite case, where the protein is a monomer in
solution (as evident from light scattering, MW determination through
centrifugation, EPR, etc.) but crystallizes as a dimer or higher multimer?
Bernie Santarsiero
--
Tommi Kajander, Ph.D.
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
P.O. Box 65 (Street address: Viikinkaari 1, 4th floor)
University of Helsinki
FIN-00014 Helsinki, Finland
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