cedric bauvois wrote:
Dear CCP4ers,
in their paper entitled " Using cryoloops for X-ray data collection from
protein crystals at room temperature: A simple applicable method" (
*Journal of Crystal Growth*
<http://www.sciencedirect.com/science/journal/00220248>
Volume 281, Issues 2-4
<http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%235302%232005%23997189997%23601824%23FLA%23&_cdi=5302&_pubType=J&view=c&_auth=y&_acct=C000026678&_version=1&_urlVersion=0&_userid=532047&md5=9a4e7b2fc158c6d2396925c79d995e3d>,
1 August 2005, Pages 592-595.), the authors present a way to mount
crystals using "a cryoloop accompanied by a glass capillary cap" (see
abstract below).
Do you know if any commercial version of such system are now available ?
Sounds like the Mi-te-gen MicroRT method - only that uses polyester capiliary
which scatters xrays less than the glass:
http://www.mitegen.com/products/micrort/micrort.shtml
Abstract: Although cryoloops are now routinely used for X-ray data
collection from protein crystals in cryocooling condition, it is still
necessary to collect X-ray diffraction data from protein crystals at
room temperature under such circumstances as to find resolution limit
and/or to avoid damage of protein crystals at cryogenic temperature
(e.g. 100 K). Here, we show that a cryoloop, which is accompanied by a
glass capillary cap to maintain humid environment of crystal in the
cryoloop, can be used not only to examine protein or non-protein
crystals but also to collect X-ray diffraction data for structural
analysis from protein crystals at room temperature. The size of cryoloop
should be carefully chosen so that the crystal does not move in the
cryoloop. This crystal mounting method can be time-saving compared to
the traditional method to mount a crystal in a glass capillary tube.
Many thanks
--
Dr. Cedric Bauvois
Cristallographie des protéines
Institut de Recherches Microbiologiques JM Wiame -IRMW
Av E. Gryzon 1, 1070 Brussels (Belgium)
tél: +32 (0)2 5273634
fax: +32 (0)2 5267273
