Hey Amit,
as your protein is tiny, maybe you can calculate a molecular replacement
model as described in Acta Cryst. (2009). D65, 169–175.
This might take longer than engineering a few methionines, though. I'd
mutate leucines.
Andreas
amit sharma wrote:
Dear All,
I have a 9-kDa protein that crystallizes well. Since there is no
structural homologue for this molecule, I intend to make Se-Met
derivative of the protein. The molecule has no Met/Cys residues in its
sequence. I wanted to know where in the sequence should I mutate, so
that the folding/crystallizability of the protein is not compromised.
Any suggestions would be of great help.
Thanks in advance,
--
Amit Sharma, Ph.D. Research Associate, Department of Biology,
University of York, YO10 5DD UK
--
Andreas Förster, Research Associate
Paul Freemont & Xiaodong Zhang Labs
Department of Biochemistry, Imperial College London
