Hi everyone, Recently, I obtained a soluble glyco-protein. Unfortunately, after I added PNGase or Endo Hf to remove the glycans, the deglycosylated protein is precipitated. Is there any method to avoid this kind of precipitation?
Thanks, Simon
Hi everyone, Recently, I obtained a soluble glyco-protein. Unfortunately, after I added PNGase or Endo Hf to remove the glycans, the deglycosylated protein is precipitated. Is there any method to avoid this kind of precipitation?
Thanks, Simon