Presumably you want Mn2+ , but you need to specify.
If so, you need to make it up fresh, and keep it at a pH below 7 if at
all possible, as it oxidizes readily.
Bis-tris will weakly chelate it and slow the oxidation process.
On Apr 6, 2009, at 11:14 AM, Matthew Alan Bratkowski wrote:
Hi.
Does anyone have experience using solutions containing manganese as
crystallization buffers (buffers, not screening well solutions)? The
protein that I am working requires manganese for activity, and I
have read
reports of related proteins crystallizing in manganese buffers. I
made a
buffer containing 3 mM manganese, that initially had a black color
then
turned to deep purple, and later almost clear. After a few weeks, the
buffer turned an orange color and contains dark manganese syrup on the
bottom. Does anyone know how to prepare a manganese buffer that is
stable
enough to be used as a protein buffer for crystallization screens?
Thanks,
Matt
