Hi Liew,
Have you ever tried paratone or some other oil? Sincerely, Li __________________________________________ Email:s...@sibs.ac.cn Institute of Biochemistry and Cell Biology Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue-Yang Road, Shanghai 200031, China Tel: +86-21-5492-1217 __________________________________________ 发件人: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] 代表 Liew Chong Wai 发送时间: 2009年4月24日 11:47 收件人: CCP4BB@JISCMAIL.AC.UK 主题: [ccp4bb] Cryo-protectant Hi all, Good day I used MPD as a cryoprotectant (20%, 30%) for my crystal. However, there is no diffraction signal at all. Without the MPD cryo, i still manage to get 5angstrom, but it has very strong ice-ring signal. I used glycerol (15%, 20%, 25% and 30%) before, but it cracked the crystal. Please advice. Thanks Liew
