Re: [ccp4bb] Cryo-protectant

[Liew Chong Wai]

Hi all
 
Thanks for your precious suggestions and ideas. 
The crystallization buffer condition is 0.1M BIS-TRIS pH 5.5, 0.2M MgCl.6H2O, 
35% PEG3350
Now, my crystal seem ok in 20% ethylene glycol, but only after a couple minutes 
of dehydration at room temperature. For sure, i will try other cryoprotectant 
that was suggested here. 
I just wondering why MPD kills the crystal.
Many thanks
 
LIEW