Hi Jacob, there are ways, but simple and fast, I don't know. You can do extraction of some purified protein with organic solvents and doing TLC. Quantitation may be harder. In the case of phospholipids you can do a phosphorous determination (with molybdenum, the protocol should be easy to retrieve online) . For regular, non-P containing lipids you may have to go back to TLC with standards. For removing the lipid, any column that binds your protein would work (metal-affinity, ion exchange), just wash with a large volume to gradually push the lipid off. Gel filtratiuon is also possible, but as you can imagine less efficient.
Bert van den Berg University of Massachusetts Medical School Program in Molecular Medicine Biotech II, 373 Plantation Street, Suite 115 Worcester MA 01605 Phone: 508 856 1201 (office); 508 856 1211 (lab) e-mail: bert.vandenb...@umassmed.edu http://www.umassmed.edu/pmm/faculty/vandenberg.cfm -----Original Message----- From: CCP4 bulletin board on behalf of Jacob Keller Sent: Tue 7/7/2009 1:01 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Lipid content/removal Dear Crystallographers, does anybody know of a simple, fast way to determine whether a protein sample contains lipid, and roughly to determine the quantity? As corollary, does anybody know of a quick way to extract/remove the putative lipid? In my case, I am working with an extremely robust/stable soluble protein which may contain bacterial lipids. Thanks for your consideration, Jacob Keller ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: j-kell...@northwestern.edu *******************************************
