Hi everybody,
I really apologize to every members of CCP4BB for not to give attention to my addressing phrase. It is truly misleading. I swear not to make such bad mistake in future. I am really ashamed for such a deed. Sincerely Debajyoti Dutta On Fri, 21 Aug 2009 21:29:23 +0530 wrote >Dear Sir, Please don't assume that all crystallographers are male. ---- Original message ---- >Date: Thu, 20 Aug 2009 16:45:55 -0000 >From: Debajyoti Dutta >Subject: Re: [ccp4bb] cyseteine modification >To: CCP4BB@JISCMAIL.AC.UK > > Dear Sir, > > Thank you all who have replied. I jast want to > enquire that if there is any option in coot to > introduce the OH bond with Cys residue as well as to > introduce the secondary peptide like bond between > carboxylic carbon with amino group of lys. > > Sincerely > Debajyoti Dutta > > On Wed, 19 Aug 2009 20:58:44 +0530 wrote > > > > Dear Debajyoti > > There is also the sulfenic acid species (-S-OH) > which is actually > the first oxidized form of sulfhydryls on the way to > sulfonic acid. However > sulfenic acids are very susceptible to further > oxidation to sulfinic and sulphonic > acids, and therefore need a protective chemical > environment to remain stable. > > See for example some previous work of ours on > sulfenic and > sulfinic forms of active-site cysteines in > glutathione reductase (Nature > Structure Biology vol 5, 267-271, 1998) and the > corrresponding pdb entries 1dnc > and 1gsn. > > Best regards > > Savvas > > ---- > Savvas Savvides > L-ProBE, Unit for Structural Biology > Ghent University > K.L. Ledeganckstraat 35 > 9000 Ghent, BELGIUM > office: +32-(0)9-264.51.24 ;mobile: > +32-(0)472-92.85.19 > Email: savvas.savvi...@ugent.be > http://www.lprobe.ugent.be/xray.html > > From: CCP4 bulletin board > [mailto:ccp...@jiscmail.ac.uk] On Behalf Of > Debajyoti Dutta > Sent: Wednesday, August 19, 2009 4:42 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] cyseteine modification > > Dear Sir, > > Is there any other oxidation states of cysteine > other than cysteine sulphinic > acid and cysteine sulphonic acid. In my protein, the > cysteine molecule is > definitely overoxidized but the electron density is > not corresponding to the > sulphinic or the sulphonic acid. The positive > density looks as if it can > accomodate only one oxygen atom and not more. > > Thank you for reply in advance. > > Sincerely > Debajyoti Dutta > > > > >E-mail message checked by Spyware Doctor > (6.1.0.447)Database version: > 6.13080http://www.pctools.com/spyware-doctor-antivirus/ > > [IMG] Phoebe A. Rice Assoc. Prof., Dept. of Biochemistry & Molecular Biology The University of Chicago phone 773 834 1723 http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123 RNA is really nifty DNA is over fifty We have put them both in one book Please do take a really good look http://www.rsc.org/shop/books/2008/9780854042722.asp
