Beyond improvement via 'chemical' means, I would heartily recommend mutagenesis for crystal packing improvement. I would be glad to help you with the latter if you're interested.
Artem > Dear All: > We have been struggling to improve the crystals shown in the attachment. > These crystals form from a protein solution 8 mg/mL (before drop), drop > sizes are 2 microL of protein + 2 microL of well solution (0.1 MES > buffer, pH 6.5, 1.8 M Ammonium sulfate with 12 mM CoCl2). > > The protein was dissolved initially in 20 mM Tris.HCl, pH 8.0 containing > 1 mM EDTA. > > Low cobalt also gives smaller crystals. These diffract up to 8 A. > > Any suggestions to improve these crystals would be most welcome. > > Subbu > > PS: We have used many additives (Hampton Research - including detergent > additives) to alter the morphology but with little success. >
