Hi Claudia
"By eye" is not a bad approximation. The trick I learnt at the JCSG (I
forget the originator, sorry) is to suck up some solution in a P2
pipette tip (the clear ones), dip it in lN2, and then hold it just above
the lN2 (where it's still cold) to check for clearness or not.
Is a good predictor especially if your loops are very small (volume is a
big factor in whether you can achieve vitrification or not).
Cheers
phx
On Tue, Sep 22, 2009 at 4:24 AM, Claudia Scotti
<claudiasco...@hotmail.com <mailto:claudiasco...@hotmail.com>> wrote:
Dear List,
Sorry for the probably silly question.
Any suggestions to test cryoconditions without X-rays or cryostream?
I'd need to freeze crystals before going to ESRF and I'm a bit
anxious. Is it enough to try to freeze the cryoconditions in
liquid nitrogen checking under the microscope (or by eye) or is
this still risky?
Thanks,
Claudia
Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di
Patologia Generale Universita' di Pavia Piazza Botta, 10 27100
Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 303673
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