Mike England wrote:
Hi all,
I will appreciate your comments on the following case:
I have two datasets from the same or identical crystals. Initially, I
refine a structure against the first data set and later on switch to
another dataset for further refinements.
Do you think, my Rfree will be biased as Rfree reflections in second
dataset may be in fact Rwork reflections in previous datasets ?
Thanks in advance,
Mike
Yes [- it will be biased, but why arent preserving the same Rfree set
from crystal 1 into the crystal 2 assignmenmt, only selecting new free
reflections at the higher resolution data from crystal 2
If you are running scala or import scaled data t make an mtz file there
is an option:
Click Ensure Free R and Copy freeR from another mtz
Eleanor
