Hi Martyn, this recipe tends to work for me...
Lysozyme: 50 mg/ml in 0.1 M Sodium Acetate pH 4.8 Reagent: 8% w/v Sodium Chloride, 0.1 M Sodium Acetate pH 4.8 Additional Reagents: Index Reagent 8, 22, 28, 31, 34, 40, 58, 59, 69, 86, 88 Mix equal amounts of lysozyme with reagent, incubate at 4 or 22 degrees Celsius. Batch or vapor diffusion works fine. ==> Direct copy/paste from http://hamptonresearch.com/experiments.aspx <== HTH and Happy Christmas. Cheers, Dave ============================ David C. Briggs PhD ============================ University of Manchester E-mail: david.c.bri...@manchester.ac.uk ============================ Twitter: @xtaldave Skype: DocDCB ============================ 2009/12/21 MARTYN SYMMONS <martainn_oshioma...@btinternet.com>: > Dear All > checking out the Lysozyme crystallization methods on the web I liked the > Rigaku Instructions that I found: > (http://www.rigaku.com/protein/crystallization.html) > > "...create a drop of 3ul lysozyme solution, and 3 ul of well solution, > respectfully, for a total drop size of 6ul..." > > So perhaps sometimes I am just not respectful enough to deserve crystals ? > > good wishes to all > regards, > Martyn > ----------------------- > Martyn Symmons > MRC-MBU Cambridge UK > 'Chan fhiosrach mur feòraich.' > Gaelic proverb - > Nothing asked, nothing learned. >
