Hi Martyn,
When it comes to micro-dialysis, you could check the following paper:
http://www.jbc.org/content/260/25/13580.full.pdf+html
(freely available, check in particular the appendix for the
micro-dialysis setup that was used, that was for heat-labile enterotoxin)
Fred.
PS I know this is not exactly answering your question but still you
might find this setup useful
MARTYN SYMMONS wrote:
Thanks to those who replied.
The humour comes of course from a slight feeling that there is
some truth in the 'respectful' version. Proteins are wonderful subtle
machines so perhaps we should be careful and thoughtful when we expose them to
the
horrors of most crystallization conditions!
Which takes me to the original point of my search - which was for a dialysis
method for lysozyme. I was trying to set up a micro dialysis format for my
protein but wanted to test it first. I am using spectrapore 8K dialysis
membrane and wondered if that would retain lysozyme enough to get crystals (I'd
like to stick with this cut-off as the physical and sealing properties of other
membranes might be different). Or if someone has a higher MW cheap alternative
protein/condition?
Best wishes - agus 'Bliadhna Mhath Ur Dhuibh Uile' as we say in gaelic.
Martyn
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Martyn Symmons
Cambridge
