Phoebe, my understanding is that power of UV illumination here is not in salt-vs-protein test (which, imho, can only be finalized by testing diffraction) but in improved contrast for protein crystal detection. I haven't used UV microscopes myself, but images provided by manufacturers are quite impressive http://www.rigaku.com/images/automation/uv-before.jpg http://www.rigaku.com/images/automation/uv-after.jpg
Of course, these images are advertisement and may be exaggerated. On the salt/protein issue, I wholeheartedly agree. Ed. On Wed, 2010-01-20 at 13:52 -0600, Phoebe Rice wrote: > Are these things really cost-effective? That is, compared to > the cost of simply posting a list of evil salts like Mg > ammonium phosphate on the wall and testing a few duds in the > x-ray beam? > Phoebe > > ---- Original message ---- > >Date: Wed, 20 Jan 2010 13:59:23 -0500 > >From: Gabriel Birrane <gbirr...@bidmc.harvard.edu> > >Subject: Re: [ccp4bb] UV microscope for screening > >To: CCP4BB@JISCMAIL.AC.UK > > > >I received this in the mail today. You may be interested. > > > >http://www.fmpproducts.com > > > >They have products for crystallography > > > > > >On Wed, 2010-01-20 at 11:28 -0500, Scott Walsh wrote: > >> Dear CCP4BB members, > >> > >> I am shopping around for a UV microscope for the lab and > would like to get some feedback > >> about peoples' experiences using UV microscopes for > screening crystals. > >> > >> Best, > >> > >> Scott > >> > >> ********************************************************* > >> Scott T. R. Walsh, Ph.D. > >> Assistant Professor > >> Center for Advanced Research in Biotechnology > >> University of Maryland Biotechnology Institute > >> Rm 3127E CARB II > >> 9600 Gudelsky Drive > >> Rockville, MD 20850 > >> email: wal...@umbi.umd.edu > >> phone: (240) 314-6478 > >> fax: (240) 314-6255- > >> > Phoebe A. Rice > Assoc. Prof., Dept. of Biochemistry & Molecular Biology > The University of Chicago > phone 773 834 1723 > http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123 > > RNA is really nifty > DNA is over fifty > We have put them > both in one book > Please do take a > really good look > http://www.rsc.org/shop/books/2008/9780854042722.asp -- Edwin Pozharski, PhD, Assistant Professor University of Maryland, Baltimore ---------------------------------------------- When the Way is forgotten duty and justice appear; Then knowledge and wisdom are born along with hypocrisy. When harmonious relationships dissolve then respect and devotion arise; When a nation falls to chaos then loyalty and patriotism are born. ------------------------------ / Lao Tse /
