It is also possible that mother liquor prevents binding (although often in such cases you would see some precipitant component in the active site.
I would generally bet on need for conformational change. And you expect to see the product complex, right? Ed. On Fri, 2010-04-09 at 11:15 +0200, Paul Lindblom wrote: > Dear Bulletin Board, > > I am trying to soak substrate into crystals of an enzyme, but so far I > can't see the substrate in the structure. Does anyone knows a program > to ensure that the entrance to the central cavity is accessibly? I > mean based on the whole crystal. I already checked the crystal packing > manually and it seems that the way is free more or less, but I find it > hard to interpret. > > Thanks in advance, > > P. -- Edwin Pozharski, PhD, Assistant Professor University of Maryland, Baltimore ---------------------------------------------- When the Way is forgotten duty and justice appear; Then knowledge and wisdom are born along with hypocrisy. When harmonious relationships dissolve then respect and devotion arise; When a nation falls to chaos then loyalty and patriotism are born. ------------------------------ / Lao Tse /
