Hello Arpit, don't you use DNAse during lysation of the cells? Or do you mean that you want to get rid of unspecific DNA and want to add some specific DNA after purification? In that case I'd understand you don't want to use DNAse.
Tim On Wed, May 12, 2010 at 06:23:56PM +0530, Arpit Mishra wrote: > ---------- Forwarded message ---------- > From: Arpit Mishra <ar...@igib.in> > Date: Wed, May 12, 2010 at 6:20 PM > Subject: regarding purification of DNA binding protein > To: ccp...@jiscmail.ac > > > hi all > i am trying to purify DNA binding protein, but i couldnt get rid of the DNA > after performing hi-trap heparin column, i have also tried high conc salt > buffer (upto 1 M NaCl) and also tried o.2 M lithium sulphate, please suggest > me some way to get rid of undesired DNA.. > > thanks > > Regards > > Arpit -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A
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