Dear all, Sorry for this silly biochemistory question. Thing is that I have a reversible epimerase and I want to mutate it into an inreversible one. However, I have been told that the ΔG of a reversible reaction is zero. Which direction the reaction goes depends only on the concentration of the substrate. So the conclusion is, A: I can mutate the epimerase into an inreversible one. But it has no influence on the reaction direction, and hence it has little mean. B: There is no way to change a reversible epimerase into an inversible one. Could somebody please give me some comment on the two conclution? Thank you all for your time. Best, Vinson
