Dear Crystallographers,
I am trying to optimize a native gel experiment of a two-protein complex,
running the smallest-detectable amount of protein component A with varying
amounts of component B.
MW Charge MW/Charge
A 22 -5 -4308
B 17 -24 -702
This experiment is partly to determine stoichiometry, but also to determine
roughly the strength of the interaction.
B definitely runs much faster than A alone, as predicted, but I am wondering
what to expect with various oligomers. Should ABB run faster or slower than
AB? What about AABB? Theoretically, AA should certainly run slower than A,
and BB slower than B, simply because the mass/charge ratio is the same, but
the overall mass is greater. But what happens when you have AAB, for
example? There must be an equation relating the mass/charge and mass (and
perhaps gel percentage) to the speed traveled in the gel--but what is the
equation?
Thanks for your consideration,
Jacob
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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
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