The rnase structure used as the $CEXAM for CCP4 is another example - a
typical coordinate set is 2sar.pdb
There one monomer binds the substrate very clearly, whilst the other is
blocked by crystal contacts.
Eleanor
ANDY DODDS wrote:
Hello,
I am solving a structure of an enzyme, which crystallises as a dimer.
We have pretty good evidence that this operates as a dimer in vitro,
also. We have an inhibitor of this enzyme, which we are keen to
visualise by X-ray methods.
We seem to have very strong density in which we can model our
inhibitor, with good stats and no negative density. However, there is
only density in one of the monomers, nothing in the other. The SG is
P212121, and although I can postulate why this may have happened (if
this is indeed what HAS happened), different solvent channel
accessibility etc., I would like to know how common this was and, if
possible, some literature regarding this, if the board would be so
kind?
Regards,
Andrew.
