Did anyone using Nano ITC low volume ( 190 ul) for protein interaction? Dear Sir or Madam: Recently, my laboratory buy a Nano ITC low volume ( 190 ul) for protein-ligand interaction or protein-protein interaction. The Nano ITC low volume (190 ul) machine is made by TA instrument. The cell material of Nano ITC low volume ( 190 ul) is 99.9999% Gold. We found that almost every protein precipitate during titration, while there is no precipitate if the protein mixed with ligand or protein in plastic tube. . In addition, the K value from Nano ITC low volume (190 ul) is just half of the standard value by CaCl2 and EDTA titration or NaHCO3 and HCl titration. Can Nano ITC low volume (190 ul) be used for measuring protein interaction? Any suggestions are very appreciated. Thanks 2010-07-23
dengzq1987
