Hi, A little danger this condition. I suggest you should firstly make sure they are protein crystals. I know it is very difficult. I suggest you can add proteases to the drops. If "crystals" dissolve, no problem, they are protein crystals.
Best Yibin 2010-08-27 yybbll 发件人： rui 发送时间： 2010-08-27 02:04:54 收件人： yybbll 抄送： 主题： Re: [ccp4bb] turn granular to crystal hi, no , I didn't use detergent. THe composition of the well buffer is just PEG4000 and MgCl2 and NaAc. On Wed, Aug 25, 2010 at 9:52 AM, yybbll <yyb...@gmail.com> wrote: Hi, Did you use detergent? It seems look like detergent crystals. 2010-08-25 yybbll 发件人： rui 发送时间： 2010-08-25 20:37:18 收件人： CCP4BB 抄送： 主题： [ccp4bb] turn granular to crystal Hi, All, I'm trying to crystallize a soluble protein and got something like granular, they are rounded shaped and not so regular and also don't have sharp edges. See the attached pic. The current condition is PEG4000 and pH around 5. How can I improve this condition? Thanks a lot.
