Hello Eric, I would try not to store a protein for 3-4 days and set up drops as quickly as possible. 3-4 days can be a long time for a sensitive protein. If you expressed too much, add 15% glycerol and store the protein at -80deg. Gel-filtrate the solution and re-concentrate before the next use.
Tim On Fri, Nov 05, 2010 at 08:40:51PM +0000, <Eric Karg> wrote: > Dear all, > > I'm working on a protein which starts to precipitate after 3-4 days of > storage at 4 degrees or room temperature. The storage buffer contains 300 mM > NaCl because at lower salt concentrations it also tends to precipitate. > Different buffers and adding glycerol did not help although this was not done > in a systematic way. Has anyone had similar experiences? Any suggestions to > overcome this problem? > > Thanks in advance! > > Eric -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A
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