Mother liquor plus 30% glycerol or 30% glucose will cryoprotect pretty
much anything, if it does not cause crystal cracking. We have had very
good general luck with 25-30% glucose, and it's easy to prepare from
your well solution or crystallization master mix. Add 150 mg of glucose
to a microcentrifuge tube, make up to the 0.5 mL mark with your
crystallization well solution, sonicate or otherwise mix thoroughly
until dissolved. You can either transfer and swish crystals in the cryo
solution, or gradually dilute your drop to 10X volume of the cryo-solution.
Cheers.
On 12/15/2010 4:13 PM, Jerry McCully wrote:
Dear All;
Recently we got some crystals from the condition #51 in the
SaltRx crystallization kit from Hampton research.
It contains 1.5M Na2HPO4 and 0.1M Tris(pH8.5). We am going to
do a test diffraction ASAP.
What cryoprotectant did you use for this condition?
Thanks a lot and have a nice holiday season!
Jinghua
--
------------------------------------------------------------------------
Roger S. Rowlett
Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: rrowl...@mail.colgate.edu
