Dear All, Recently I am trying to express a glycoprotein in Drosophila S2 cells. However, the expression yield is every low and most of the protein expressed aggregate. I was suggested making new constructs. But I am wondering if anyone here can give me some suggestions to improve it beyond that since I am new to glycoprotein? All your input will be greatly appreciated! Regards, Min
- [ccp4bb] low expression and aggregation of protein m zhang