Thanks, Lin. I am sorry for being short of information. I was actually not sure
what kind of information is needed. Well, the cells were grown at 27 degree in
Insect-Xpress. I tried to grow cells both in shaker and stationery flask.
Anything else I can try?
Best,
Min
Date: Sat, 22 Jan 2011 21:57:02 +0100
Subject: Re: [ccp4bb] low expression and aggregation of protein
From: yyb...@gmail.com
To: mzhang...@hotmail.com
CC: CCP4BB@jiscmail.ac.uk
Actually, it is very difficult to say something just based on your simple
information. You should give more informations about your work, for example,
temperature, cuture, etc.
Best,
Lin
On Fri, Jan 21, 2011 at 8:12 PM, m zhang <mzhang...@hotmail.com> wrote:
Dear All,
Recently I am trying to express a glycoprotein in Drosophila S2 cells. However,
the expression yield is every low and most of the protein expressed aggregate.
I was suggested making new constructs. But I am wondering if anyone here can
give me some suggestions to improve it beyond that since I am new to
glycoprotein? All your input will be greatly appreciated!
Regards,
Min
