If you make the images available, maybe I can take a look and try to use d*TREK. You will need to be selective about the spots used in indexing. Most programs can add or delete spots manually. You may wish to make the X-ray beam smaller and try to expose only a small volume of one of the crystals. Any "joined" or "overlapped" volume probably has both lattices.
However, if the lattices are not well separated, there may be no point in persuing this. You may be able to try seeding to grow non-joint crystals. That is, you may wish to concentrate your efforts in the wet lab rather than on this dubious diffraction data. _____ From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of pravinkumar jagtap Sent: Friday, February 11, 2011 10:07 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Regarding Indexing of data from two crystals in a loop Hi to all, I have crystals which grow as joint crystals i.e two crystals are joined to one another. I tried to seperate the two and collect dataset but the resolution is not good( probably I am harming the crystals during seperation). Also I collected a dataset with the joined crystals in the loop and I got resolution up to 3.5 A on synchrotron. But when I am trying to index this dataset, the XDS is failing . I also tried to use mosflm but it is also giving warning message that the unit cell parameters are too inaccurate. And this all seems to be because of the two crystals in the loop. Can anyone suggest any strategy which I should take for indexing this dataset. Thanks, Pravin Kumar
