Hi Sebastiano, we are very happy with the SEC columns from Wyatt coupled to our FPLC/MALLS machine. http://www.wyatt.com/solutions/hardware/sec-columns-for-multi-angle-light-sc attering.html
The separation range is (more or less) comparable to GE standard spdx 200/75/superose 6, and the columns are rock solid. The quality of separation is excellent in analytical runs, we routinely use them loading sample volumes of 10-25 microliters at concentrations around 1mg/ml. We did not encounter reproducibility problems/column damage so far with buffers at pH in the 6-8.5 range (8.5 is actually the high pH limit recommended by Wyatt). HTH Best, Federico Forneris, PhD ------- Crystal and Structural Chemistry Bijvoet Center for Biomolecular Research - Utrecht University Padualaan 8 3584 CH UTRECHT - The Netherlands Tel. +31 (0) 30 253 2868 - Fax +31 (0) 30 253 3940 http://www.crystal.chem.uu.nl/group-gros/ -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Sebastiano Pasqualato Sent: martedì 8 marzo 2011 14:04 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] [OT] which column to use in SLS/MALS instruments Dear all, I was wondering if somebody could help me out by suggesting the "best" column to be used in a Static Light Scattering (I guess it would be the same for a Multi Angle Light Scattering) instrument. We were suggested using a silica-based column, with very high separation properties, but it seems that these columns are highly sensitive to (even slightly) basic pH's. Even running the column in PBS, it looks like injecting samples at pH 8.0 ruins the column resin, making it unusable. On the other hand, GE Healthcare columns would require a huge amount of material to be loaded. What are you guys using in your instruments? Thanks a lot in advance for the feedback, best, ciao Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit IFOM-IEO Campus Cogentech - Consortium for Genomic Technologies via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5172 fax +39 02 9437 5990
