So from where does Wyatt outsource their columns? JPK
On Tue, Mar 8, 2011 at 10:49 AM, Sally Pham Thanh Van <sally.pha...@gmail.com> wrote: > I should say clearer that I did a comparison between GE (Superdex 200 > 10/30) and the Wyatt column of the same separation range on AKTA > connected with MALS. The Wyatt column gave better resolution for size > exclusion chromatography as well as signal-noise ratio for light > scattering. So I think it is worth for you to try it. They offer > money-back guarantee. > > Best wishes, > Sally. > > On Tue, Mar 8, 2011 at 3:36 PM, Sally Pham Thanh Van > <sally.pha...@gmail.com> wrote: >> Dear Sebastiano, >> >> From my experiences, Wyatt columns are the best. >> >> Best wishes, >> Sally. >> >> On Tue, Mar 8, 2011 at 2:03 PM, Sebastiano Pasqualato >> <sebastiano.pasqual...@gmail.com> wrote: >>> Dear all, >>> I was wondering if somebody could help me out by suggesting the "best" >>> column to be used in a Static Light Scattering (I guess it would be the >>> same for a Multi Angle Light Scattering) instrument. >>> We were suggested using a silica-based column, with very high separation >>> properties, but it seems that these columns are highly sensitive to (even >>> slightly) basic pH's. Even running the column in PBS, it looks like >>> injecting samples at pH 8.0 ruins the column resin, making it unusable. >>> On the other hand, GE Healthcare columns would require a huge amount of >>> material to be loaded. >>> What are you guys using in your instruments? >>> Thanks a lot in advance for the feedback, >>> best, >>> ciao >>> Sebastiano >>> >>> -- >>> Sebastiano Pasqualato, PhD >>> Crystallography Unit >>> IFOM-IEO Campus >>> Cogentech - Consortium for Genomic Technologies >>> via Adamello, 16 >>> 20139 - Milano >>> Italy >>> >>> tel +39 02 9437 5172 >>> fax +39 02 9437 5990 >>> >> > -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program cel: 773.608.9185 email: j-kell...@northwestern.edu *******************************************