> what does the twinning analysis by Phenix tell? > if you haven't done it yet i would think you should run the processed > data through phenix Xtriage and see > what is the twinning analysis tell you. > It also is a good program to solve your space group issues. > I think you might need a mtz file or sca as the input.
xtriage had indicated a presence of merohedral twin, with twin law = h,-h-k,-l > first of all if you can collect a better data set. > is non-merohedral and partial hemihedral twinning possibility? > highly unlikely. chances are you have many small plates of crystals > growing > close by and you are collecting data on a bunch of crystals stacked > up.just > guessing. > It was a single crystal. atleast morphologically it was not looking like multiple crystal. > I remember, at an ACA meeting there was a talk discussing twinning issues. > The gist was: he did process a data set with so complicated twinning > issues and > it took several months to get a solution. > later they were successful in getting better crystals > and solved the same protein structure in a different space group > and the electron density map obtained from > both looked so drastically different the final interpretation of the > active site function > was so different. > question number 1: if the data of such complication is solved how much > information > is obtained? > question number 2: is it possible to get better data than this? > question number 3: if there is no other way to improve your crystals i > will > keep on working on such issues. > Fortunately I have got another data, I was able to get a solution for the twinned structure, but was difficult to refine, When I compared twinned structure with those two structures it does not vary much. > Hope this helps > cheers > Padayatti PS > With Regards Kavya -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean.
