you can do amino acid analysis on your pure protein, using a commercial or academic service - I hope these are still around. You should only need to do this once, then relate the result to your A220, BCA and Bradford assays. Mark
Quoting Arpit Mishra: > hello everybody > > i am working on the protien which dont have any aromatic residue i do fplc > other purification using 220 absorption, but i want to quantitate protein > precisely i have tried using BCA nd bradford but both methods quantification > is not matching,,so any one is having sum idea how to quantitate it > precisely > > thanks in advance for your valuable suggestion.. > > > Arpit Mishra > Mark J van Raaij Laboratorio M-4 Dpto de Estructura de Macromoléculas Centro Nacional de Biotecnología - CSIC c/Darwin 3, Campus Cantoblanco 28049 Madrid tel. 91 585 4616 email: mjvanra...@cnb.csic.es
