Dear Ramanuj Banerjee, from the attached picture it is difficult to make out N- and C-terminus of your protein and it looks like two closed molecules intertwined- which would indeed be very surprising.
However, if you think there is space for the molecules to form such a homodimer (either in solution or upon formation of the crystal), why should it not be possible? It seems like a beautiful conformation. Cheers, Tim On Tue, Apr 26, 2011 at 08:37:29AM +0100, Ramanuj Banerjee wrote: > Dear All, > I have solved a protein structure (experimentally phased) with 1 > molecule in > the asymmetric unit at 2.22 A (high resolution). The present R factor is .22 > and R free .27 with Ramachandran favoured >98% and R and R free are > decreasing with refinement.The problem is: when the pdb is opened in pymol > and symmetry mates generated, the upper part of the molecule shows to be > intertwined with the symmetry molecule (attached .jpg), but there are no > clashes in between the two.The electron density is so very fine that no > alternative choices of chain flow are available.All the processes starting > from phasing and refinement have been done in Phenix.Is such a thing > possible ? > -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A
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