Yes, its surely possible. If there are no clashes start thinking if this dimer is the physiological molecule in solution.
Check probability that this dimer is stable in solution in Pisa - ebiFold If so, check first with size exclusion columns if it behaves like dimer in solution, preferably use a MALLS setup if available. Ssaxs can confirm it's shape. AUC is handy for kD. And if its a dimer then of course mutate the interface and assay ... Have fun! Tassos Sent from my iPhone On 26 Apr 2011, at 09:37, Ramanuj Banerjee <ramanuj.baner...@saha.ac.in> wrote: > Dear All, I have solved a protein structure (experimentally phased) with 1 > molecule in the asymmetric unit at 2.22 A (high resolution). The present R > factor is .22 and R free .27 with Ramachandran favoured >98% and R and R free > are decreasing with refinement.The problem is: when the pdb is opened in > pymol and symmetry mates generated, the upper part of the molecule shows to > be intertwined with the symmetry molecule (attached .jpg), but there are no > clashes in between the two.The electron density is so very fine that no > alternative choices of chain flow are available.All the processes starting > from phasing and refinement have been done in Phenix.Is such a thing possible > ? > <a copy.jpg>
