Hi, Yes indeed, as Matt was kind enough to point out, we do have the HC1 installed at station I911-3 of the MAX-II ring and you are welcome to make a rapid access application. One advantage of MAX-lab might be that our wiggler beam is more merciful to the crystals at room temperature than the undulator beams at the ESRF! There are a few free days left in our spring schedule:
http://cassiopeia.maxlab.lu.se/index/organiser-app Good luck Derek ___________________________________________________________________ Derek Logan tel: +46 46 222 1443 Associate Professor fax: +46 46 222 4692 Dept. of Biochemistry and Structural Biology mob: +46 76 8585 707 Centre for Molecular Protein Science www.cmps.lu.se Lund University, Box 124, 221 00 Lund, Sweden www.saromics.com On May 2, 2011, at 8:11, Matthew BOWLER wrote: > Dear Israel, > as Martin pointed out we have a device here at the ESRF/EMBL, the > HC1b, that produces a stream of air with a precisely controlled RH at > the sample position that we have used with some success to monitor the > effects dehydration has on diffraction quality. The same device is also > available at Diamond, Max-Lab and, I believe, BESSY. The example you > describe is a classic example of the sort of system that will usually > benefit from controlled dehydration. Depending on the size and > concentration of the LMW PEG you are using you have probably reduced the > "RH" surrounding your crystal by ~10%. The best thing to do now is > repeat these experiments using the HC1b to really define the changes in > the lattice of your crystals and find the optimum dehydration conditions > for your crystals. At the ESRF the device can be requested for any > experimental session (just click the check box on the A form) and I > presume that this will be similar at the other synchrotrons. > > As well as the reference describing the device we have recently > published a further description of typical experimental conditions and > some successful applications: > > http://dx.doi.org/10.1016/j.jsb.2011.03.002 > > And the ESRF webpage is here: > > http://www.esrf.fr/UsersAndScience/Experiments/MX/About_our_beamlines/ID14-2/HC1b > > Good luck! Matt > > > On 01/05/2011 19:32, Israel Sanchez wrote: >> Hi folks, >> >> >> I am currently impressed by the efficiency of dehydration treatments >> over the diffraction capacity of our crystals in one particular >> condition. Without any treatment the crystals seldom diffract to >> 20-30A but in our last synchrotron trip the very same crystals, after >> been incubated with increasing concentration of low molecular weight >> PEGs diffracted to 6A. >> >> I was wondering if anyone has studied these effects in a systematic >> way. Does anyone on the ccp4bb knows references or has any >> experience/pseudo-religious believes that do not care to share with >> the community about this particular topic? >> >> >> Thank you very much in advance!!!! >> >> >> -- >> Israel Sanchez Fernandez PhD >> Ramakrishnan-lab >> MRC Laboratory of Molecular Biology, >> Hills Road, Cambridge, CB2 0QH, UK >> >> > > -- > Matthew Bowler > Structural Biology Group > European Synchrotron Radiation Facility > B.P. 220, 6 rue Jules Horowitz > F-38043 GRENOBLE CEDEX > FRANCE > =================================================== > Tel: +33 (0) 4.76.88.29.28 > Fax: +33 (0) 4.76.88.29.04 > > http://go.esrf.eu/MX > http://go.esrf.eu/Bowler > ===================================================
