I always try sugars for everything. There is a cryocrystallography webinar at rigaku.com with embedded videos on how to do this. 50% to 100% saturated sugar (sucrose, glucose, trehalose, sorbitol, et al.) in reservoir buffer is usually what I try. :)
-----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Chris Ulens Sent: Thursday, May 26, 2011 5:27 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] recommendation for ammonium dihydrogen phosphate cryo Hi, I would like to get recommendations for a proper cryo solution for a crystallization hit from the Hampton crystal screen Ammonium di- hydrogen phosphate 0.4M. I tried increasing glycerol up to 30% with the same ammonium phosphate concentration or increasing glycerol up to 30% in the presence of 1.3M ammonium phosphate. Both gave iced up drops (I only tried quick and dirty tests by dipping a cover glass in liquid nitrogen). Thank you. -Chris
