---------- Forwarded message ---------- From: Dilip Kumar <dku...@igib.in> Date: Mon, Jun 13, 2011 at 11:21 AM Subject: Fwd: Regarding Sel-Met containing proteing crystallisation To: atulsingh21...@gmail.com
---------- Forwarded message ---------- From: Dilip Kumar <dku...@igib.in> Date: Sat, Jun 11, 2011 at 6:09 PM Subject: Regarding Sel-Met containing proteing crystallisation To: CCP4BB@jiscmail.ac.uk Dear all I have got protein crystals,crystallisation condition (LiCl, PEG and HEPES) .Crystals of native protein have been successesfully reproduced but when i tried to reproduce these crystals with protein having Met replaced by Sel-Met, i could not get any crystal.I tried crystallisation trials by varying pH and PEG concentration and diffferent drop ratio but i could not get any hit.Please suggest me what could be the possible reasons behind it? And also suggest the other variables that i can try ? thanks With Regards -- Dilip Tiwari Graduate Student Structural Biology Unit Institute of Genomics & Integrative Biology Delhi-07 -- Dilip Tiwari Graduate Student Structural Biology Unit Institute of Genomics & Integrative Biology Delhi-07
