How about hydrophobic interaction chromatography? JPK
On Fri, Jun 24, 2011 at 12:22 PM, Shiva Bhowmik <gene1...@gmail.com> wrote: > Amonium sulfate precipitation? Considering the fact that you tried to > seperate tubulin contamination both by charge and mass difference amonium > sulfate pption may work. > > Shiva > > On Thu, Jun 23, 2011 at 4:05 AM, Seungil Han <shan06...@gmail.com> wrote: >> >> All, >> I am sorry that this is off topic. >> My target protein expressed in insect cell expression system is eluted >> with tubulin contamination. We have tried ion exchange & sizing column to >> get ride of tubulin from our target protein for structural studies. >> >> Has anyone had similar problem? Any suggestion? >> >> Thanks in advance. >> Seungil >> >> >> Seungil Han, Ph.D. >> Pfizer Inc. >> Eastern Point Road, MS8118W-228 >> Groton, CT 06340 >> Tel: 860-686-1788, Fax: 860-686-2095 >> Email: seungil....@pfizer.com > -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program cel: 773.608.9185 email: j-kell...@northwestern.edu *******************************************
