Hi Alenxander, Thanks for the reply. Here's the details on the unit cell parameters
[image: image.png] [image: image.png] [image: image.png] Thank you. :) Regards, Fairolniza On Mon, Jul 11, 2011 at 2:47 PM, Alexandre OURJOUMTSEV <sa...@igbmc.fr>wrote: > Dear Ferrol,**** > > ** ** > > Could you let us know the unit cell parameters for both these crystals ? > (did I miss them somewhere in your previous mails?). I wonder if in fact > this is not the same packing with minor variation. > > Sacha**** > > ** ** > > *De :* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *De la part > de*ferrol shariff > *Envoyé :* dimanche 10 juillet 2011 10:10 > *À :* CCP4BB@JISCMAIL.AC.UK > *Objet :* [ccp4bb] Same protein, different molecule numbers per ASU**** > > ** ** > > Hello and good day to everyone! :)**** > > ** ** > > I have some general questions on crystallography work. I hope you don't > mind giving me some ideas. > > I have solved my lipase protein both ground-grown crystals and space-grown > crystals with good resolutions (1.4A and 2.2A). They are the same protein > from the same source, same purification methods, and produced crystals from > the same crystallization conditions (except the gravity part). > > From the data, it shows that both of them belong to the same space group > P212121. But they have different number of molecule per asymmetric unit. > Ground crystal= 1 molecule/ASU, Space crystal= 2 molecules/ASU. At the > moment i have problem explaining this issue. Is it normal to have such > results? Same protein with different number of molecule/ASU? > > I've been trying to get some references on this matter but so far i don't > really get anything that can directly explain it. Furthermore, do i need to > relate this with the gravity effect? > > I hope you don't mind sharing some experiences on crystallography > especially regarding this matter. > > Thank you very much > > -- > FAIROLNIZA > > **** > -- FAIROLNIZA "The advantage of the emotions is that they lead us astray, and the advantage of science is that it is not emotional" -Oscar Wilde, The Picture of Dorian Gray, 1891
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