They must represent a different packing - the volume of Shape 1 is 10% > than 2*Vol-Shape2
But it isnt uncommon to get different forms - check your symmetry contacts (PISA will do that) and see how the two forms pack. eleanor On Mon, 11 Jul 2011 18:09:52 +0800, ferrol shariff <ferrol2...@gmail.com> wrote: > Hi Alenxander, > Thanks for the reply. Here's the details on the unit cell parameters > > [image: image.png] > > [image: image.png] > > [image: image.png] > > Thank you. :) > > Regards, > > Fairolniza > On Mon, Jul 11, 2011 at 2:47 PM, Alexandre OURJOUMTSEV > <sa...@igbmc.fr>wrote: > >> Dear Ferrol,**** >> >> ** ** >> >> Could you let us know the unit cell parameters for both these crystals ? >> (did I miss them somewhere in your previous mails?). I wonder if in fact >> this is not the same packing with minor variation. >> >> Sacha**** >> >> ** ** >> >> *De :* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *De la part >> de*ferrol shariff >> *Envoyé :* dimanche 10 juillet 2011 10:10 >> *À :* CCP4BB@JISCMAIL.AC.UK >> *Objet :* [ccp4bb] Same protein, different molecule numbers per ASU**** >> >> ** ** >> >> Hello and good day to everyone! :)**** >> >> ** ** >> >> I have some general questions on crystallography work. I hope you don't >> mind giving me some ideas. >> >> I have solved my lipase protein both ground-grown crystals and >> space-grown >> crystals with good resolutions (1.4A and 2.2A). They are the same protein >> from the same source, same purification methods, and produced crystals >> from >> the same crystallization conditions (except the gravity part). >> >> From the data, it shows that both of them belong to the same space group >> P212121. But they have different number of molecule per asymmetric unit. >> Ground crystal= 1 molecule/ASU, Space crystal= 2 molecules/ASU. At the >> moment i have problem explaining this issue. Is it normal to have such >> results? Same protein with different number of molecule/ASU? >> >> I've been trying to get some references on this matter but so far i don't >> really get anything that can directly explain it. Furthermore, do i need >> to >> relate this with the gravity effect? >> >> I hope you don't mind sharing some experiences on crystallography >> especially regarding this matter. >> >> Thank you very much >> >> -- >> FAIROLNIZA >> >> **** >>
